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Lecturer(s)
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Ševčík Juraj, prof. RNDr. Ph.D.
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Lemr Karel, prof. RNDr. Ph.D.
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Barták Petr, prof. RNDr. Ph.D.
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Bednář Petr, doc. RNDr. Ph.D.
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Course content
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Separation analytical methods- preconcentration techniques; theory of chromatographic process; gas chromatography; liquid chromatography; supercritical fluid chromatography; electromigration techniques; hyphenation of separation and spectral techniques. Optical and spectral methods-optical emission spectrometry, plasma spectroscopy, AAS, atomic fluorescent spectroscopy, X-ray spectroscopy; molecular absorption spectrometry - UV/VIS, IR, NMR, EPR, Raman, Mössbauer spectrometry; luminescent methods; spectropolarimetry; methods of electron spectroscopy (ESCA, Auger and other); mass spectrometry. Electroanalytical methods-potentiometry in discontinual and continual systems, equilibrium potentiometry; polarographic and voltametric techniques; chronopotentiometry; titration with polarized electrodes; coulometry, voltametric and coulometric analysers; conduction methods. Applied analysis - taking and preparation of samples of inorganic and organic materials, conversion of samples into solution; analysis of metallic alloys, silicates, water and soil samples; analysis of environmental pollutants, analytical chemistry in agricultural laboratories, analysis of foodstuff, analysis of biological samples of animal and plant origin; determination of structure and identification of organic compounds.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Exam
- 80 hours per semester
- Attendace
- 20 hours per semester
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Learning outcomes
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Student discovers state of the art in theory, technical development and application of instrumental methods in analytical chemistry. Understanding theory and using knowledge concerning instruments student is able to discuss optimization of methods, to interpret data, and to use them in a context of a solved problem. He/she criticaly evaluates and compares advantages and disadvatagesof methods. Student is able to select a method suitable for an analytical task. The following areas are discussed (concerning a topic of disserattion thesis): 1) Separation analytical methods - preconcentration techniques; theory of chromatographic process; gas chromatography; liquid chromatography; supercritical fluid chromatography; electromigration techniques; hyphenation of separation and spectral techniques. 2) Optical and spectral methods - emission methods (optical emission spectrometry, flame spectrometry, atomic fluorescence spectrometry, X- ray fluorescence, fluorimetry, phosphorimetry); absorption methods (atomic absorption spectrometry, molecular absorption spectrometry in UV/VIS, IR, Mössbauer spectroscopy); nuclear magnetic resonance spectroscopy, electron paramagnetic resonance; scattering (turbidimetry, nephelometry, Raman spectroscopy); polarized light (polarimetry, circual dichroism); methods of electron spectroscopy (XPS, Auger), electron microscopy. 3) Mass spectrometry. 4) Electroanalytical methods - potentiometry; polarographic and voltametric techniques; chronopotentiometry; titration with polarized electrodes; electrogravimetry; coulometry; conduction methods. 5) Applied analysis - sampling and sample treatment (inorganic and organic materials), conversion of samples into solution; structural analysis and identification of organic compounds; analysis of metals, metallic alloys, silicates, water and soil samples; analysis of environmental pollutants; analytical chemistry in agricultural laboratories; analysis of foodstuff; analysis of biological samples of animal and plant origin; pharmaceutical anaylsis; analysis of objects of cultural heritage.
To compare with critical view advantages and disadvantages of instrumental methods, to select a suitable method for a given purpose, to interpret data.
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Oral exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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Churáček J. a kol. (1990). Analytická separace látek, 1. vyd.. Státní nakladatelství technické literatury, Praha.
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Popl, M., Kubát, J. (1986). Separace látek, 3. přeprac. vyd.. VŠCHT Praha.
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Skoog D. A., Holler F. J., Nieman T. A. (1998). Principles of instrumental analysis, Edition. Saunders College Publishing, Philadelphia, USA.
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