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Lecturer(s)
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Barták Petr, prof. RNDr. Ph.D.
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Bednář Petr, doc. RNDr. Ph.D.
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Course content
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Actual topics in development and applications of dynamic separation methods: theory, techniques and applications of separation techniques, microcolumns separation techniques, multidimensional techniques and hyphenation with spectral (MS, FTIR, FTNMR, ...) and electrochemical methods, off-line techniques, preparative techniques, fast analysis and screening analysis of large sample arrays, trace analysis, preconcentration and derivatization procedures (including "on-column" techniques), technical news, current applications (analysis of biological systems, drugs and metabolites, biopolymers, food analysis, environmental analysis, syntetic polymers,...)
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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TActual topics in development and applications of dynamic separation methods: theory, techniques and applications of separation techniques, microcolumns separation techniques, multidimensional techniques and hyphenation with spectral (MS, FTIR, FTNMR, ...) and electrochemical methods, off-line techniques, preparative techniques, fast analysis and screening analysis of large sample arrays, trace analysis, preconcentration and derivatization procedures (including "on- column" techniques), technical news, current applications (analysis of biological systems, drugs and metabolites, biopolymers, food analysis, environmental analysis, syntetic polymers,...)
Explain up- to- date novelties in separation science, compare methods, choose the proper method for a given purpose
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Student performance
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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Aktuální práce v časopisech Electrophoresis, Journal of Chromatography , Journal of Separation Science, Chromatographia...
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Firemní literatura.
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Aguilar, M. I. (Ed.). (2004). HPLC of Peptides and Proteins: Methods and Protocols. Humana Press, Totowa.
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Albert, K. (Ed.). (2002). On-Line LC-NMR and Related Techniques. John Wiley & Sons, Chichester.
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Ardrey, R. E. (2003). Liquid Chromatography - Mass Spectrometry: An Introduction. John Wiley & Sons, Chichester.
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Braithwaite, E., Smith, F. J. (1999). Chromatographic Methods. Kluwer Academic Publishers, Dordrecht, Boston, London.
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Deyl, Z., Mikšík I., Tagliaro F., Tesařová E. (1998). Advanced Chromatographic and Electromigration Methods in BioSciences. Journal of Chromatography Library 60, Elsevier Amsterodam.
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Gjerde, D. T., Hanna, C. P., Hornby, D. (2002). DNA Chromatography. Wiley-VCH, Weinheim.
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Holčapek M., Byrdwell W.C. (2017). Handbook of Advanced Chromatography/Mass Spectrometry Techniques. Academic Press and AOCS Press.
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Jennings, W., Mittlefehldt, E., Stremple, P. (1997). Analytical Gas Chromatography. Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto.
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Li, S. F. Y. (1993). Capillary Electrophoresis. Journal of Chromatography Library 52, Elsevier Amsterodam.
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Mitra, S. (Ed.). (2003). Sample Preparation Techniques in Analytical Chemistry. John Wiley & Sons, Hoboken.
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Niessen, W. M. A. (1999). Liquid Chromatography-Mass Spectrometry. Marcel Dekker, New York.
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Wilson, I., Poole, C., Cooke, M. (2000). Encyclopedia of separation science. Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto.
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