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Lecturer(s)
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Havlíček Vladimír, prof. Ing. Dr.
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Course content
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Contents: 1. Introduction: Mass spectrometry in the analysis of organic molecules 2. Desorption techniques for the analysis of small polar molecules and large biomolecules 3. New modern high-resolving power mass analyzers: orbitrap and cyclotron 4. Desorption electrospray and jet dissociation for the analysis of tissues and whole blood 5. Biomarker discovery: Surface Enhanced Laser Desorption Ionization 6. Metabolomics 7. Surface Plasmon Resonance: interactions studies 8. Mass Microscopy and mass imaging: direct approach to biomarkers 9. Diagnostics of selected disorders
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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Contents: 1. Introduction: Mass spectrometry in the analysis of organic molecules 2. Desorption techniques for the analysis of small polar molecules and large biomolecules 3. New modern high-resolving power mass analyzers: orbitrap and cyclotron 4. Desorption electrospray and jet dissociation for the analysis of tissues and whole blood 5. Biomarker discovery: Surface Enhanced Laser Desorption Ionization 6. Metabolomics 7. Surface Plasmon Resonance: interactions studies 8. Mass Microscopy and mass imaging: direct approach to biomarkers 9. Diagnostics of selected disorders
Compare advantages and disadvantages of particular methods of biomarkers analysis using MS techniques, choose the suitable method for a given purpose, interpret results
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Oral exam, Written exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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(2007). International Journal of Mass Spectrometry, Special Issue on Imaging Mass Spectrometry, Vol. 260.
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Holčapek, M. (editor). (2001). Spojení vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie. Spektroskopická společnost Jana Marca Marci a Univerzita Pardubice, Pardubice.
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Jegorov, A., Hajduch, M., Sulc, M., Havlicek, V. (2006). Nonribosomal cyclic peptides: specific markers of fungal infections. J. Mass Spectrom., 41: 563.
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Kinter, M.,Sherman N. E. (2000). Protein Sequencing and identification using tandem mass spectrometry. John Wiley and sons, New York.
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Skládal, P., Macholán, L. (1997). Biosensory - Současný stav a perspektivy. Chem. Listy 91; 105-113.
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Snyder, A. P. (2000). Interpreting protein mass spectra. American Chemical Society and Oxford University Press, Washington D.C.
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