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Lecturer(s)
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Fryčák Petr, doc. RNDr. Ph.D.
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Course content
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UV/VIS spectrometry. Colours of compounds, composition of colours. Molecular orbitals, electron transitions types of molecules and ions. Absorption spectrum, influence of solvent, choice of solvent. Lambert-Beer's law and its limitations. Determining the stoichiometry of metal- complexes, equilibrium constants and structures of organic compound. Luminescence spectroscopy. Fundamental of luminescence, singlet and triplet states, Franck-Condon principle. Relaxation of molecule, fluorescence and phosphorescence, thermo luminescence, chemiluminescence. Yield and extinction of luminescence. Luminescence volumetric analysis, fluorescence indicators. Refractometry and interferometry. Refractive index, Snell's law, Lorenz molar refractivity. Nephelometry and turbidimetry. Scattering of light, kind of scattering. Principles, choice of methods, determination of concentration, applications. Infrared and Raman spectroscopy. Vibrations and rotations of molecules, kind of vibrations. Rules in IR spectroscopy. Dispersion and FT spectrometer. Special techniques in IR - external and internal reflectance. Dissimilarities of Raman and IR spectroscopy. Raman scattering. Applications. Nuclear magnetic resonance. Spin of nucleus, precess motion, energy of spin transitions, populations of nuclei, resonance condition. Pulse NMR, relaxation, shielding, chemical shift, spin-spin interaction, spin-lattice time, decoupling. Interpretation of NMR spectra - number of signals, chemical shift, intensity and shape of signal, spin-spin interaction constant. Modern methods of NMR, 2D NMR. Electron paramagnetic resonance (EPR).
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 34 hours per semester
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Learning outcomes
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The main objective of this lecture is to acquaint students with the techniques of molecular spectrometry.
Define the main concepts, describe the main approaches to identification and detemination of substances using spectral methods
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Prerequisites
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Successfully completed study of chemistry at the B.S. level.
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Assessment methods and criteria
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Mark, Oral exam
To demonstrate sufficient knowledge of the subject at the exam.
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Recommended literature
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" Freeman R.: Magnetic resonance in chemistry and medicine. Oxford University Press, 2003.
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" Friebolin H.: Basic One- and Two-Dimensional NMR Spectroscopy. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2005..
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" Garaj. J., Bustin D., Hladký Z.: Analytická chemia. Alfa/SNTL, Bratislava 1987..
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" Harris D. C., Bertolucci M. D.: Symmetry and spectroscopy. Dover Publications, Inc., New York, 1989..
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" Harvey D.: Modern Analytical Chemistry, DePauw University, 2000.
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" Jandera P.: Molekulová spektroskopie v organické analýze. Univerzita Pardubice, Pardubice 1999..
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" Kalous V. a kol.: Metody chemického výzkumu. SNTL/Alfa, Praha 1987..
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" Stužka V.: Instrumentální metody chemické analýzy. IV. Vibrační spektroskopie organických molekul., UP Olomouc, 1976. II. vydání - UP Olomouc, 1982. III. vydání - UP Olomouc, 1993..
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" Stužka V.: Spektrální identifikace organických látek. Příklady, tabulky, UP Olomouc 1997..
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" Stužka V.:Instrumentální metody chemické analýzy. III. Jaderná magnetická rezonance., UP Olomouc, 1982. II. vydání - UP Olomouc, 1993..
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" Volka K. a kol.: Analytická chemie I. VŠCHT, Praha1995..
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" Zýka J. a kol.: Analytická příručka, 2. díl. SNTL/Alfa, Praha 1988..
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