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Lecturer(s)
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Havlíček Vladimír, prof. Ing. Dr.
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Kuzma Marek, Ing. Ph.D.
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Course content
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Common interpretation approaches for conventional and product ion mass spectra good for low molecular weight organics. Per analogiam method, spectra or biopolymers. Electrospray and matrix-assisted laser desorption/ionization mass spectrometry, desorption electrospray, rapid evaporation/ionization mass spectrometry. Accurate mass and extreme mass resolving power analyzers (ion cyclotron resonance Fourier transform mass spectrometry, orbitrap), ion mobility mass spectrometry. Miniaturization. Collision-induced dissociation, electron transfer dissociation, electron capture dissociation. Analysis of peptides and identification or proteins: sample prep, mass determination, deconvolution, peptide fragmentation, peptide mapping, post-translation modifications, protein conformation, molecular interactions, proteomics, database search, protein quantitation. MS3D approaches, hydrogen-deuterium exchange, chemical crosslinking. Surface-enhanced mass spectrometry. Analysis of nucleotides/nucleosides, saccharides, lipids. Mass spectrometry imaging in medicine. Metabolomics and proteomics in infectious and tumour disease diagnostics. Practical sessions: data collection on private samples (diploma thesis) on FTICR, TOF-TOF. Nuclear magnetic resonance spectroscopy and electron microscopy demonstrations.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Although mass spectrometry has celebrated 100 years' anniversary, it still represents quite quickly growing field. The subject "Clinical mass spectrometry" covers all important recent technical and application trends. It accentuates both the practical aspects (data collection and spectra interpretation) and clinical applications. During the lectures, fundamentals, newest technologies and interpretation strategies will be reviewed. Basic knowledge acquisition will be guaranteed for students coming from diverse study fields. Ion formation mechanisms, dissociation techniques and product ion mass spectra will be discussed during the lectures. Intraoperative mass spectrometry, imaging mass spectrometry in medicine, multimodal imaging, ion mobility, MS3D techniques and other recent tools will be reviewed. Hands-on sessions with software tools (CycloBranch, mMass, etc.) will be organized.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
The knowledge of basics of organic, analytical chemistry and biochemistry is recommended.
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Recommended literature
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A. Škríba, J. Houšť a V. Havlíček. Chemické Listy 114 (2), 119-125 Iontová cyklotronová rezonance s Fourierovou.
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F. Tureček. Chemické Listy 114 (2), 126-132 Doplňkové metody v hmotnostní spektrometrii.
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F. Tureček. Chemické Listy 114 (2), 89-95. Chemie iontů v plynné fázi: základní pojmy.
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K. Lemr a L. Borovcová. Chemické Listy 114 (2), 101-105 Elektronová ionizace.
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K. Lemr a L. Borovcová. Chemické Listy 114 (2), 96-100 Vznik iontů v hmotnostní spektrometrii: ionizace.
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L. Škultéty, J. Novák a V. Havlíček. Základy interpretace hmotnostních spekter 145, Chemické Listy 114 (2), 145-154.
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M. Sadílek. Chemické Listy 114 (2), 133-144 Úvod do tandemové hmotnostní spektrometrie.
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M. Volný. Chemické Listy 114 (2), 113-118 Hmotnostní analyzátor doby letu.
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V. Havlíček a F. Tureček. Chemické Listy 114 (2), 106-112 Kvadrupólový analyzátor a iontové pasti.
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