| Course title | NMR Spectroscopy for Organic Chemistry and Biochemistry |
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| Course code | ACH/ANMR |
| Organizational form of instruction | Lecture + Seminar |
| Level of course | Master |
| Year of study | not specified |
| Semester | Winter |
| Number of ECTS credits | 3 |
| Language of instruction | Czech |
| Status of course | Compulsory-optional, Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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NMR spectroscopy is one of the most important spectral methods for identification and elucidation of molecular structure. During the last 50 years it became an advanced technique suitable not only to study structure of molecules in liquid or solid state but also for research of dynamic behavior in liquids. It makes NMR a unique method capable to follow the molecular events on atomic scale in a biological system under physiological conditions. It allows a deep insight into the complexation of drugs with their receptors, interaction of proteins between each other and with DNA, or RNA. 1. Nuclear magnetic resonance - a phenomenon, basic terms. 2. Generation of NMR signal, practical aspects of NMR experiment, Fourier transformation, basic pulse sequence (spin echo). 3. Relaxation, NOE and its importance, chemical exchange. 4. NMR parameters - chemical shift, interaction constant J and signal multiplicity, dipolar interaction D, NOE. 5. Molecular structure characterization of organic compounds: a. 1H NMR, homonuclear correlation - J-resolved, COSY, TOCSY b. 13C NMR, heteronuclear correlation - HMQC, HSQC, HMBC c. selective and band-selective experiments. 6. Spectra interpretation. Conformation studies by NMR - application of conformation dependent NMR parameters. 7. Biomacromolecules- nucleic acids and proteins, stable isotope labeling - 13C, 15N a 2H. 8. NMR biomacromolecules - basic strategy. 9. Non-covalent interaction of biomacromolecules with ligands.
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| Learning activities and teaching methods |
Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
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| Learning outcomes |
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To acquaint students with the possibilities and use of NMR spectroscopy for org. substance.
Explain principles, describe the solutions, solve the problems and interpret data acquired by means of NMR |
| Prerequisites |
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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| Assessment methods and criteria |
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Student performance
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| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
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| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2019) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Analytical Chemistry (2021) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Winter |