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Lecturer(s)
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Ševčík Juraj, prof. RNDr. Ph.D.
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Petr Jan, doc. RNDr. Ph.D.
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Course content
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Applications of electromigration techniques Slab gel electrophoresis. PAGE, 2D electrophoretic techniques, blotting. Semipreparative techniques, autofocusing, demonstration of practical application. Capillary electrophoresis. Electrolyte systems for capillary electrophoresis. Electrochromatography. Indirect detection, on-line derivatization techniques, off-line and on-line preconcentration techniques. Enantiomeric separation. Separation of biopolymers (DNA, low-molecular peptides, oligosaccharides). Separation of inorganic ions, hydrophobic and lipophilic compounds. HPCE in clinical practices, forensic, toxicological, pharmaceutical and food industry. Home-made electrophoretic devices, miniaturization and progress of HPCE.
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Learning activities and teaching methods
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Lecture
- Attendace
- 13 hours per semester
- Homework for Teaching
- 13 hours per semester
- Preparation for the Course Credit
- 34 hours per semester
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Learning outcomes
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The aim is to become familiar with the procedures for application of electromigration techniques.
Define and describe the basic relations and laws for the migration of charged species in electric field. Describe the main possibilities for influencing the ionic mobilities to achieve the effective separation. Discuss the changes of mobilities of ionic species by means of chiral selectors, complexing agents, organic solvents, etc. Apply the theoretical knowledge to ionic species migration to design electrophoretic separation.
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Oral exam
colloquium and discussion on optional two questions
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Recommended literature
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Ferenčík M., Škárka B.: Biochemické laboratórne metódy. ALFA Bratislava 1981. Deyl. Z., Mikšík I., Tagliaro F., Tesařová E.: Advanced Chromatographic and Electromigration Methods in BioSciences. Journal of Chromatography Library 60, Elsevier Amsterodam, 1998. Chrambach A., Dunn M.J., Radola B.J. (eds.): Advances in Electrophoresis 6, VCH Verlagsgesellschaft Weinheim, 1993. Chrambach A., Dunn M.J., Radola B.J. (eds.): Advances in Electrophoresis 7, VCH Verlagsgesellschaft Weinheim, 1994. Rothe G.M.: Electrophoresis of Enzymes. Springer Verlag Berlin, 1994. Sorensen H., Sorensen S., Bjergegaard C., Michaelsen S.: Chromatography and Electrophoresis in Food Analysis. MPG Books Ltd, Bodmin Cornwall 1999.
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1. S.M. Palfrey, Clinical Applications of Capillary Electrophoresis, Humana Press, (1999)..
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2. K. D. Altria, Capillary Electrophoresis Guidebook: Principles, Operation, and Applications, Humana Press (1996).
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