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Lecturer(s)
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Pauk Volodymyr, Mgr. Ph.D.
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Papoušková Barbora, RNDr. Ph.D.
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Ševčík Juraj, prof. RNDr. Ph.D.
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Course content
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Analytical chemistry in context with other natural sciences. Classification of qualitative and quantitative methods of chemical analysis. The common steps in quantitative analysis - the choice of method, sampling, representative sample, preparation of laboratory sample, decomposition and dissolving of sample, elimination of interferences, calibration and measurement of sample (analytical signal). The interpretation of analytical signal. Analytical reactions. Qualitative inorganic analysis (the Okáč´s system) and organic analysis (CHONX, functional group). Gravimetry and volumetry. Basic principle and application of electrochemical methods (potentiometry, polarography, conductometry) spectroscopic methods (RGT, AAS, UV-VIS, IR, NMR, MS - structural analysis) and sepration methods (GC, HPLC, CE). Biochemical analytical methods (immunoanalysis of low-molecular compounds). Analysis of real samples. Preconcentration and pre-separation techniques. Demostration of develompment of analytical method. The examples of some analysis in forensic, hygiene, metallurgic and research laboratory.
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Learning activities and teaching methods
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Lecture
- Attendace
- 39 hours per semester
- Homework for Teaching
- 26 hours per semester
- Preparation for the Course Credit
- 12 hours per semester
- Preparation for the Exam
- 13 hours per semester
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Learning outcomes
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The aim is to become familiar with the sample preparation in applied analytical chemistry and gain practical skills
Describe the basic principles of analytical chemistry. List the criteria for choosing the right analytical methods. Describe the principles of the most usable instrumental analytical techniques. Apply knowledge to instrumental analysis of biological samples
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Mark
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Recommended literature
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Pánek P., Dombek V. (1994). Základy analytické chemie pro studenty ekologie.
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Šimek J., Bekárek V. (1985). Základy analytické chemie.
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