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Lecturer(s)
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Frébortová Jitka, doc. RNDr. Ph.D.
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Course content
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Scope, emergence and development of molecular biotechnology. Microbial diversity and microorganisms with uses in biotechnology. Recombinant DNA technology, principles of gene expression in prokaryotic and eukaryotic cells. Large-scale production of proteins from recombinant microorganisms. Production of protein and nucleic acids therapeutics, recombinant vaccines. Synthesis of enzymes, small biological molecules, antibiotics and biopolymers by recombinant microorganisms. Microbial insecticides (Bacillus thuringiensis toxin, baculoviruses). Bioremediation and biomass utilization.
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 40 hours per semester
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Learning outcomes
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To introduce modern industrial, biomedical, pharmaceutical and agricultural applications of microbial and cell cultures.
Student should be able to describe basic principles of molecular biotechnology, to describe principles of production of commercial products and therapeutics using genetically engineered microorganisms and cell cultures, and to describe the use of genetically engineered microorganisms for insect pest control,remediation and biomass utilization.
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Prerequisites
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To know fundamentals of biochemistry, microbiology and molecular biology.
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Assessment methods and criteria
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Mark, Oral exam
Consistent with lecture content. Student should be able to discuss individual topics.
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Recommended literature
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Glick B.R., Pasternak J.J., Patten Ch. (2010). Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition. ASM Press, USA, 2010. ISBN 978-1-55581-498-4.. Washington DC.
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