Course title | Practice in Proteomics |
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Course code | LRR/CVPRO |
Organizational form of instruction | Exercise |
Level of course | Master |
Year of study | not specified |
Semester | Summer |
Number of ECTS credits | 3 |
Language of instruction | Czech |
Status of course | Compulsory |
Form of instruction | Face-to-face |
Work placements | This is not an internship |
Recommended optional programme components | None |
Lecturer(s) |
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Course content |
1. Extraction of a soluble fraction of proteins from a biological material; 1D or 2D SDS/PAGE gel electrophoresis; Commassie Briliant Blue gel staining. 2. Cutting of selected protein spots from a gel, in-gel digestion with trypsin and/or other proteases (e.g. chymotrypsin); extraction of digested peptides. 3. Preparation of peptide extracts for mass spectrometry analysis - Zip Tip purification on a C18 sorbent, a chemical modification of peptides with two different reagents; mass spectrometry analysis of purified and modified peptides and protein identification after database search (MALDI-TOF, ESI-QTOF, MASCOT DATABASE).
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Learning activities and teaching methods |
Laboratory Work
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Learning outcomes |
To learn practical methods used in proteomic.
Student should be able to (after attending the course): - apply basic proteomic methods for analysis of proteins in a biological material - analyse collected raw data (protein identification, determination of molecular mass, study of post-translational protein modification and others) - write an experimental protocol and discuss of obtained results |
Prerequisites |
Basic experiences with laboratory work, knowledge from proteomic lectures.
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Assessment methods and criteria |
Written exam
100% attendance, completion of all experimental tasks |
Recommended literature |
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Study plans that include the course |
Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
---|---|---|---|---|
Faculty: Faculty of Science | Study plan (Version): Experimental Biology of Plants (2021) | Category: Biology courses | 1 | Recommended year of study:1, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Experimental Biology (2015) | Category: Biology courses | 1 | Recommended year of study:1, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | 1 | Recommended year of study:1, Recommended semester: Summer |