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Lecturer(s)
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Kubínek Roman, doc. RNDr. CSc.
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Course content
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Light microscopy - method of phase contrast, UV and IR microscopy, fluorescent microscopy, polarization microscopy, interference microscopy (Nomarski interference contrastm Hoffman modulation contrast), confocal laser microscopy, optical scanning near-field microscopy Electron microscopy - transmission electron microscopy, scanning electron microscopy, low-voltage electron microscopy, electron microscopy with high resolution, electron microscopy with optional vacuum (biological application) Scanning probe microscopy - scanning tunneling microscopy, atomic force microscopy, magnetic force microscopy, electrostatic force microscopy, lateral force microscopy, scanning capacity microscopy, scanning temperature microscopy, scanning optical near-field microscopy, methods related to the group of methods of scanning probe microscopy
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
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Learning outcomes
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Overview of used modern microscopic methods - modern light microscopy, transmission and scanning electron microscopy, scanning probe microscopy
The course focused on gaining the ability to understand the problem. Explain the nature and ability to interpret data, to distinguish and classify of microscopic methods.
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Prerequisites
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Basic cource of physics
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Assessment methods and criteria
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Student performance
Knowledge of the topics, ability to discuss on the topics in wider contexts
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Recommended literature
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Kubínek, R. (1986). Experimentální metody biofyziky-rastrovací elektronová mikroskopie.. UP Olomouc.
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Kubínek, R., Mašláň, M., Vůjtek, M. (2002). Mikroskopie skenující sondou. UP Olomouc.
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Kubínek, R. Materials in PDF form. (available online at http://apfyz.upol.cz/ucebnice/prehled.html).
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Murphy, D.B. (2001). Fundamentals of Light Microscopy and Electronic Imagine. Wiley.
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Reimer, L. (1998). Scanning Electron Microscopy.Physics of Image Formation and Microanalysis.. Springer.
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Vůjtek, M., Kubínek, R., & Mašláň, M. (2012). Nanoskopie. V Olomouci: Univerzita Palackého.
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