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Lecturer(s)
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Valárik Miroslav, Mgr. Ph.D.
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Doležal Karel, Mgr. Dr.
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Plačková Lenka, Mgr. Ph.D.
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Course content
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Biochemical and molecular markers - mechanisms, major types, typical examples of their use in taxonomy, <i>in-vitro</i> cultures, somaclonal variation. Protein markers and Isozymes. Polyacrylamide gel (PAGE) electrophoresis. Specific and non-specific protein staining. Chemotaxonomy - principles, major groups of compounds used, methods of their analysis (gas and liquid chromatography, mass spectrometry), data analysis. Development and characterization of molecular markers. Genetic and Physical mapping; mapping populations. Molecular Cytogenetics. Methods of physical positioning of DNA sequences on chromosomes and interphase nuclei. Utilization of methods based on hybridization of DNA molecules and polymerase chain reaction (PCR) as sources of molecular markers and data analysis. Molecular markers as a tool for clone development and gene characterization.
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Learning activities and teaching methods
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Lecture
- Preparation for the Exam
- 20 hours per semester
- Attendace
- 28 hours per semester
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Learning outcomes
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Biochemical and molecular markers - mechanisms, major types, typical examples of their use in taxonomy, <i>in-vitro</i> cultures, somaclonal variation.
Student should be able to (after attending the course): - Characterise and classify biochemical and molecular markers. - Compare principles and application of various biochemical and molecular markers. - Apply properly biochemical and molecular markers in the research.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark
in extent of the lectures
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Recommended literature
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Lewin, B. (2000). Genes VII.. Oxford University Press, Oxford.
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Singh, R.J. (1993). Plant Cytogenetics. CRC Press, Boca Raton.
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Singh, R.J. (2003). Plant Cytogenetics. CRC Press, Boca Raton.
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Šípal, Z., Anzenbacher, P., Peč, P., Pospíšil, J., Růžička, I. (1992). Biochemie. SPN, Praha.
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Tanksley , D.S., Orton, T.J. (eds.). (1983). Isozymes in Plant Genetics and Breeding, Part A. Elsevier, Amsterdam - Oxford - New York.
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Wagner, R.P. et al. (1993). Chromosomes. A Synthesis. Wiley-Liss, New York.
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