Partial tasks include preparation of biological material - bees, bee tissues or bee products for biochemical analysis. The preparation involves maintaining bees in laboratory and inducing stress reactions. In the next step, students apply their knowledge from many scientific fields, as they will focus on the biochemical analysis of parameters of immune reactions, physiological processes, antioxidant reactions and others. Students will use chromatographic, electromigration, immunochemical, molecular biological and other methods for the analysis of partial immune or physiological pathways, for example the detection of factors of humoral immunity, determination the damage by oxidative stress, diagnosis of diseases by microbiological techniques, etc. The course also focuses on the analysis of bee products, students will try common methods for determining the composition and quality of bee products, eg. honey. Syllabus 1) In vitro maintaining bees and larvae and induction of stress reactions 2) Analysis of humoral components of immunity 3) Quantification of lipid damage 4) Detection of bee pathogens 5) Analysis of honey quality 6) Antimicrobial activity of bee products Teaching implementation: Teaching will take place in blocks after the end of the summer semester in the range of approximately 2 calendar weeks. Unfortunately, it is not possible to increase the capacity (12 participants) of the subject above the set limit.
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Bankova, V., Bertelli, D., Borba, R., Conti, B. J., da Silva Cunha, I. B., Danert, C.. Zampini, C. (2016). Standard methods for Apis mellifera propolis research. Journal of Apicultural Research, 1-49. doi:10.1080/00218839.2016.1222661.
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Bogdanov, S., Martin, P., Lullmann, C., Borneck, R., Flamini, C., Morlot, M.. Ivanov, T. (1997). Harmonised methods of the European Honey Commission. 28, 1-59.
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Carreck, N. L., Andree, M., Brent, C. S., Cox-Foster, D., Dade, H. A., Ellis, J. D.. Vanenglesdorp, D. (2013). Standard methods for Apis mellifera anatomy and dissection. Journal of Apicultural Research, 52(4). doi:10.3896/ibra.1.52.4.03.
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Dietemann, V., Ellis, J. D., & Neumann, P. (Eds.). (2013). COLOSS beebook (Volume I, Standard methods for Apis mellefera research). Northern Bee Books.
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Dietemann, V., Ellis, J. D., & Neumann, P. (Eds.). (2013). COLOSS beebook (Volume II, Standard methods for Apis mellifera pest and pathogen research). Northern Bee Books.
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Hartfelder, K., Bitondi, M. M. G., Brent, C. S., Guidugli-Lazzarini, K. R., Simoes, Z. L. P., Stabentheiner, A. . Wang, Y. (2013). Standard methods for physiology and biochemistry research in Apis mellifera. Journal of Apicultural Research, 52(1). doi:10.3896/ibra.1.52.1.06.
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Williams, G. R., Alaux, C., Costa, C., Csaki, T., Doublet, V., Eisenhardt, D.. Brodschneider, R. (2013). Standard methods for physiology and biochemistry research in Apis mellifera. Journal of Apicultural Research, 52(1).
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