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Lecturer(s)
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Luhová Lenka, doc. RNDr. Ph.D.
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Course content
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Proteins, their composition and structure. Saccharides and polysaccharides, their structure and metabolism. Biosynthesis of saccharides. Glycolysis, metabolism of glycogen, citrate cycle, pentose phosphate cycle. Metabolism of lipids and amino acids. Metabolism of nucleotides. Enzymes, enzyme kinetics, inhibition. Coenzymes. Substrate specificity. Isoenzymes. Enzyme catalysis. Regulation of enzyme activity. Biochemistry of regulation. Experimental methods of biochemistry. Enzyme activity assays. Separation methods. Chromatography, electrophoresis and centrifugation of biological molecules.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Exam
- 50 hours per semester
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Learning outcomes
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Get possession of complex and substantial knowledges of metabolic patheways, reciprocal interconnection and regulation.
Associate information, interpret experimental data, solve problems, report on novelties in biochemistry
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Prerequisites
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Successful graduated studium of Bachelor of Science and Magister of Science in Biochemistry.
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Assessment methods and criteria
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Oral exam
the candidate is expected to conduct scientific discussion in broader context of the whole discipline.
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Recommended literature
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Berg, J.M., Tymoczko, J.L. and Streyer, L. (2007). Biochemistry. Freeman and company, New York.
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D. Voet, J. G. Voet and Ch. W. Pratt. (2006). Fundamentals of Biochemistry . New York, USA.
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