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Lecturer(s)
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Šebela Marek, prof. Mgr. Dr.
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Course content
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Amino acids and peptides. Peptide sequencing and synthesis. Nomenclature of peptides. Naturally occurring peptides: hormones, antibiotics and toxins. Proteins. Structures of proteins. Allostery. Methods of protein molecular mass determination. Enzymes. Specificity and relationship to reaction equilibrium and activation energy. Factors influencing enzyme activity. Enzyme kinetics. Michaelis-Menten equation, significance of the constant Km, methods of enzyme activity assay. Activation of enzymes. Reversible and irreversible inhibitions. Types of reversible inhibitions. Classification and nomenclature of enzymes. Glycolysis and alcohol fermentation. Conversions of pyruvate. Pyruvate dehydrogenase complex. Citrate cycle. Pentose phosphate cycle, regulation of pentose and hexose levels. Metabolism of disaccharides and glycogen. Gluconeogenesis. Regulation of gluconeogenesis and glycolysis. Lipids. Metabolism of fats and fatty acids. The role of carnithine, degradation of saturated, unsaturated and branched fatty acids. Metabolism of ketonic compounds and their significance. Biosynthesis of fatty acids. Degradation of amino acids, relationship to tricarbonic acid cycle. Urea cycle. Calvin cyclus. Photorespiration. Components of nucleic acids. Biosynthesis and degradation of pyrimidine and purine nucleotides. DNA and RNA.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Exam
- 50 hours per semester
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Learning outcomes
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The subject represent a review of the knowledge at the master-study level and further extends some topics.
successful completion will verify and confirm knowledge of the discipline acquired during studies.
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Prerequisites
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The candidate is expected to conduct scientific discussion in broader context of the whole discipline.
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Assessment methods and criteria
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Oral exam
Exam in extent of the lecture.
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Recommended literature
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Voet a kol. (2006). Fundamentals of Biochemistry. New York.
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