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Lecturer(s)
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Šebela Marek, prof. Mgr. Dr.
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Frébort Ivo, prof. RNDr. CSc., Ph.D.
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Course content
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Biotechnology progress towards genetically modified organisms (GMO). Biological safety and ethics. Genetically modified microorganisms and their applications. Genetically modified plants. Genetically modified animals. Techniques of genetic transformation of different classes of organisms. Transformation vectors and their construction. Promoters used for functional expression of inserted genes. Selection markers and principles of selection of transformed organisms. Detection of genetically modified organisms in marketed food and food stuff. Expected future directions of development of GMO technologies.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Exam
- 50 hours per semester
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Learning outcomes
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Obtaining advanced knowledge about GM organisms.
Explain main aspects in the field of GMO, review methods.
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Prerequisites
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The course is intended for doctoral students in Biochemistry.
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Assessment methods and criteria
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Dialog
The candidate is expected to conduct scientific discussion in broader context of the whole discipline.
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Recommended literature
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Káš, J. (2004). Geneticky modifikované organismy - současnost a perspektivy. VŠCHT Praha.
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Moo-Young M. (2011). Comprehensive Biotechnology, 2nd edition, Vol. 1-6, Pergamon, 2011.
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Poltronieri P, Hong Y. Applied plant genomics and biotechnology, Elsevier, 2015.
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Slater, A., Scott, N.W., Fowler, M.R. (2003). Plant biotechnology: The genetic manipulation of plants. Oxford University Press, Oxford, UK.
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