Course: Advanced Biochemical and Biotechnological Methods

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Course title Advanced Biochemical and Biotechnological Methods
Course code KBC/PBM
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 3
Language of instruction Czech, English
Status of course Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Bergougnoux-fojtik Véronique Hélene, Ph.D.
  • Zalabák David, Mgr. Ph.D.
  • Škrabišová Mária, Mgr. Ph.D.
  • Lenobel René, Mgr. Ph.D.
Course content
Typically the program includes the following topics: Methods of determination of protein structure. Introduction to metabolomics. Introduction to proteome analysis. Glycomics and lipidomics. Biosensors. Introduction to confocal and electron microscopy. Fundamentals of cloning and genetic engineering. Principles and applications of PCR. Cell cultures and production of recombinant proteins. Genetic transformation of cereals. Genetically modified organisms.

Learning activities and teaching methods
Lecture
  • Attendace - 26 hours per semester
  • Preparation for the Exam - 55 hours per semester
Learning outcomes
Obtaining specific advanced knowledge on modern trends in biochemitsry and related disciplines. Lectures given by experts on selected topics.
Define main concepts, ideas and principles of biochemical methods, outline procedures and strategies of biochemical methods.
Prerequisites
The course is intended for master students in Biochemistry and other chemistry and biology students.

Assessment methods and criteria
Written exam

Obtaining at least 20 out of 30 points in a written test (answers to 15 questions, duration 1.5 hour) is needed to pass the exam.
Recommended literature
  • Boyer R. (2012). Biochemistry Laboratory. Upper Saddle River, New Jersey.
  • Wilson and Walker's. (2018). Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press; 8th edition.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Analytical Biochemist (2024) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biochemistry (2022) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Molecular and Cell Biology (2021) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Inorganic and Bioinorganic Chemistry - specialization in Bioinorganic Chemistry (2021) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Bioorganic Chemistry and Chemical Biology (2020) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Bioinformatics (2021) Category: Informatics courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Analytical Chemistry (2021) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biotechnology and Genetic Engineering (2019) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter