| Course title | Immunochemistry |
|---|---|
| Course code | KBC/IMCH |
| Organizational form of instruction | Lecture |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 3 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional, Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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Introduction, Immunology and immunochemistry, Importance of immune system for live, Branches of immunology, The subject of immunochemistry investigation, Antigens, The substances that induce a specific immune response, Antigenic determinants, Survey of antigens, Immunoglobulins, The classes of antibodies and their properties, Basic structure of antibody molecules, Antigen-binding site of the antibody molecule, Isotype, allotype, idiotype, Antigen-antibody interactions, The equilibrium constant, The affinity, The avidity, The strength of the antibody-antigen interaction, Precipitation reactions, Agglutination reactions, Production of antibody, Immunisation, Adjuvants, Theory of antibody production, Production of polyclonal antibody, Preparation of pure immunoglobulins, Purification and characterisation, Fraction precipitation, Electrophoretic methods, Ionex and gel chromatography, Hydrophobic chromatography, Affinity chromatography, Monoclonal antibodies, Production of monoclonal antibodies and their properties and utilization, Precipitation reactions in fluids, Precipitation immunochemical methods, Immunonephelometry, Immunoturbidimetry, Agglutination reactions, Immunodiffusion (simple and double), Immunoelectrophoresis, Immunoblotting, Radioimmunoassay, Enzyme immunoassay, Detail of procedures, Evalutions, Fluorescence immuno-assay, Chemiluminescence, Antibody biosensors, Synthesis of conjugates (hapten-protein, hapten-enzyme, antibody-enzyme), Binding of antibody on quartz crystal microbalance, Utilization of antibodies in cytology and histochemistry, Mapping of enzymes in plant cell by antibodies, Utilization of antibodies and immunologic and immunochemical technique in medicine, Questions about preparation and utilization of antibodies in research.
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| Learning activities and teaching methods |
Lecture
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| Learning outcomes |
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Basic immunologic notion. Survey of immunomethods.
Define main concepts, describe main approaches and methods in immunochemistry. |
| Prerequisites |
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Successeful competion of basic course: Fundamentals of Biochemistry.
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| Assessment methods and criteria |
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Mark, Written exam
Written exam lasts for 1 hour and has 20 questions. Threshold for passing the exam: 65 % points A 40-38 points; B 37-35 points; C 34-32 points; D 31-29 points; E 28-26 points, F 25-0 points |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2022) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Chemistry (2019) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biochemistry (2022) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Chemistry - Analytical Specialist (2021) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Bioinorganic Chemistry (2021) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Summer |