|
Lecturer(s)
|
-
Béres Tibor, Mgr. Ph.D.
-
Tarkowski Petr, doc. RNDr. Ph.D.
-
Ćavar Zeljković Sanja, Ing. Ph.D.
-
Kaczorová Dominika, Mgr.
-
Vrobel Ondřej, Mgr.
|
|
Course content
|
Students will be familiarized with methods of isolation of secondary metabolites from various plant organs and their identification and quantitation by classic separation and specral methods. Selected secondary metabolites will be analysed by intrumental methods - liquid chromatography, capillary electrophoresis and mass spectrometry.
|
|
Learning activities and teaching methods
|
Demonstration, Laboratory Work
- Preparation for the Course Credit
- 30 hours per semester
- Attendace
- 50 hours per semester
- Homework for Teaching
- 30 hours per semester
|
|
Learning outcomes
|
The aim of the practicals is getting skills in isolation, purification and final analysis of plant secondary metabolites.
After completing the course the student should be competent with common methods of isolation, purification and final analysis of low-molecular-weight compounds.
|
|
Prerequisites
|
Practical skills in organic, physical and analytical chemistry.
|
|
Assessment methods and criteria
|
Oral exam, Student performance
Participation, submissions of all lab reports.
|
|
Recommended literature
|
-
Gullberg, J. (2005). Metabolomics. Doctoral thesis, (UPSC, Umea, Sweden).
-
Hazenkamp, A. (2014). Úvod do léčby konopím. samonáklad.
-
Jahodář L. (2011). Farmakobotanika: semenné rostliny.. Univerzita Karlova v Praze, Nakladatelství Karolinum.
-
M. Wink. Annual Plant Rewiews, Volume 39 , Functions and Biotechnology of Plant Secondary Metabolites, John Wiley & Sons, 2010.
-
Nicolaou, K. C., Montagnon, T. (2008). Molecules that Changed the World. Wiley-Vch GmbH&Co. KGaA.
-
Pavela, R. (2011). Biopesticidy.. Kurent.
-
Séquin, M. (2017). The Chemistry of Plants and Insects, Plants Bugs and Molecules. Royal Society of Chemistry.
-
S.J. Cutler, H.G. Cutler. (1999). Biologically active natural products: Pharmaceuticals. CRC Press.
|