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Lecturer(s)
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Jedelská Tereza, Mgr. Ph.D.
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Luhová Lenka, doc. RNDr. Ph.D.
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Course content
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Historical aspects. Basic properties of enzymes as catalysts. Cofactors. Nomenclature and classification of enzymes. Localisation and purification of enzymes. Structure of enzymes. Methods of study of active site. Covalent modification of enzymes. Mechanisms of enzyme actions. Enzyme kinetics - the Michaelis-Menten equation, Michaelis constant, reaction order, enzyme units, turnover number. Effect of pH on enzyme activity. Effect of temperature on enzyme activity. Enzyme reaction with one, two or three substrates. Enzyme specificity and regulation - allosteric regulation, covalent modification. Enzyme inhibition. Methods of determination of enzyme activity. Immobilization of enzymes. Control of metabolic pathways. Organized enzyme systems. Enzymes in the cell. Clinical aspects of enzymology. Enzyme technology.
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Learning activities and teaching methods
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Lecture, Work with Text (with Book, Textbook)
- Preparation for the Exam
- 60 hours per semester
- Attendace
- 26 hours per semester
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Learning outcomes
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Obtaining of knowledge from enzymology.
Define main concepts, describe main approaches in enzymology
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Prerequisites
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Elementary knowledge of biochemistry.
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Assessment methods and criteria
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Written exam
Written exam, 1 hour, 11 tasks; 20 points A 55-51 (100-91 %) B 50-45 ( 90-81 %) C 44-39 ( 80-71 %) D 39-33 ( 70-60 %) E 32-28 ( 59-50 %) F: < 28 (< 50 %)
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Recommended literature
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Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry. New York, USA.
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Bisswanger H. (2011). Practical Enzymology. Weinheim, Wiley.
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Bisswanger, H. (2019). Practical Enzymology. Wiley-VCH Verlag GmbH.
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Bugg T. D. H. (2012). Intoduction to enzyme and coenzyme chemistry 3rd Ed.. WestSussex UK.
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Cornish-Bowden A. (2012). Fundamentals of Enzyme Kinetics,Weinheim, Germany,. Weinheim, Germany.
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Editors: Munishwar Nath Gupta, Vladimir N. Uversky. (2022). Structure and Intrinsic Disorder in Enzymology (2022). Elsevier.
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Macholán L., Barthová J., Kučera I. (1994). Enzymologie MU Brno, 1994. MU Brno.
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Marangoni A. G. (2003). Enzyme Kinetics a Modern Approach. New Jersey.
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Price N. C., Stevens L. (2000). Fundamentals of enzymology. New York, USA.
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Purich D. L. (2010). Enzyme Kinetics: Catalysis and Control. London.
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