| Course title | Bioenergetics |
|---|---|
| Course code | KBC/BEN |
| Organizational form of instruction | Lecture |
| Level of course | Master |
| Year of study | not specified |
| Semester | Winter |
| Number of ECTS credits | 3 |
| Language of instruction | Czech, English |
| Status of course | Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Biological membranes - basic types of energy-transducing membranes, membrane transport. Thermodynamics of biochemical processes - biochemical systems and energy flow, free Gibbs energy and entropy, chemical, electrochemical and redox potentials. Chemiosmotic flow of protons - chemiosmotic theory, protonmotive force, proton stechiometry, respiration control, proton reverse flow. Mitochondrial respiratory chain - components, electron flow and proton translocation, stechiometry, artificial electron acceptors and donor, coupling and uncoupling of phosphorylation, heat production in brown adipose tissue. Production of oxygen radicals and oxidative stress, plant alternative oxidase. Bacterial respiratory chains - P.denitrificans, E.coli, H.pylori, Nitrobacter, Thiobacillus, methanogenesis. The origin of protonmotive force in photosynthesis - capture of radiation energy, transfer of excitation energy. Photosynthetic bacteria - Rhodobacter, photosynthetic reaction centres, photosynthesis of green algae and plants, photosystem II and I, cyclic electron transport. Bacteriorhodopsin and halorhodopsin as ion pumps. ATP synthase - F1.Fo-ATPasa, subunits, enzymology, regulation. Transport of metabolites and ions across membranes - mitochondrial transporters and transports mechanisms of bacteria. Mitochondria and disease - oxidative stress, apoptosis and necrosis, reperfusion cell injury, neurodegenerative disorders, mitochondrial genome and genetic disorders. Regulation of ion permeability as signal mechanism - neurotransmitters, excitable membranes, transmission of neuronal excitation. Energy transduction in organism movement - molecular motors, cellular cytoskeleton, bacterial movement, mechanism of muscle contraction. Experimental methods of bioenergetics.
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| Learning activities and teaching methods |
Lecture, Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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| Learning outcomes |
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To obtain wider knowledge and understanding on the concepts, mechanisms and regulation of energy tranduction within living cells and organims.
Understanding the basic principles and mechanisms of energy conversion in cells and organisms, the ability to describe the bioenergetic conditions in the cell, identify energy sources for important biological processes. |
| Prerequisites |
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Students are expected to have the knowledge and skills obtained from successful completion of the course KBC/BCH Fundamentals of Biochemistry.
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| Assessment methods and criteria |
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Mark, Written exam
The subject ends with an exam in the form of a written test. The test contains 20 questions, each of which is evaluated with a maximum of 2 points for the correct answer and its justification. Test grading scale: F: 0-19, E: 20-23, D: 24-28, C: 29-32, B: 33-36, A: 37-40. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Winter |
| Faculty: Faculty of Science | Study plan (Version): Analytical Biochemist (2024) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Winter |