| Course title | Practicals in Biochemistry |
|---|---|
| Course code | KBC/BCHCU |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Identification of amino acids. Determination of the isoelectric point of casein. Determination of the presence of glutamic acid in food flavorings. Qualitative and quantitative analysis of carbohydrates in sweeteners. Determination of proteins by the Bradford method in a tube and microplate arrangement. Isolation of amine oxidase from pea seedlings and determination of its activity. Determination of the Michaelis constant of the interaction of pea amine oxidase with putrescine and the inhibition constant of the selected inhibitor. Activity, substrate specificity and pH optimum of enzymes. Isolation of Ri-plasmid DNA from the bacterial strain Agrobacterium rhizogenes R15834 by the alkaline lysis method. Preparation of lipid fractions from egg yolk. Separation methods. Gel chromatography. Agarose electrophoresis. Determination of the isoenzyme representation of plant peroxidases by discontinuous electrophoresis in a polyacrylamide gel under native conditions.
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| Learning activities and teaching methods |
Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
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| Learning outcomes |
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Practical realization of basic experimental method from biochemisty.
Apply and practice fundamental biochemical procedures and methods, apply methods in the practice. |
| Prerequisites |
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Basic knowledge of biochemistry.
KBB/BZP ----- or ----- KFC/BZP ----- or ----- KFC/BZP0 ----- or ----- KFC/BZP1 ----- or ----- KFC/BZP2 |
| Assessment methods and criteria |
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Student performance
A pair of students solving a given task submits one common protocol in printed form (max. total of 11 tasks, i.e. 11 protocols). Prepared protocols must be handed in the following exercise! The student will receive 5 points for each report submitted on time, which is correctly drawn up. If, on the other hand, he does not submit the protocol or its correction within the proper deadline, 5 points will be deducted. If the corrected report is returned to the student, 2 points will be deducted. If it is found that the protocol was described (eg the reported results will not match the data in the workbook) the exercise is invalid and the student cannot receive credit. 3) During the exercise, the student's readiness will be checked through a test (5 questions from the theoretical and practical part of the task + 1 numerical example). - A well-prepared student gets 6 points for the exercise (the maximum number of points earned is 60). - If 3-4 points are obtained from the test, the leader is obliged to examine the student orally, in case of ignorance of the given topic, the student is expelled from the exercise. If the type of questions in the test did not suit the student, but he orients himself in the given topic, then he continues with the exercises. The number of points from the test remains the same. - A student who gets 2 or less points from the test does not continue with the exercise and must replace it. Due to insufficient preparation, the exercise can only be repeated once per semester. - If the student does not meet the condition of obtaining at least 70% points after the first half of the exercise (i.e. the first six tasks), he writes a control test in the 8th week. If he does not write the control test at min. 70%, does not continue the exercise and must register for it the following year. If students complete all 11 exercises under the given conditions as part of their regular studies, they can get a maximum of 115 points. Students who complete a smaller number of exercises due to national holidays, planned practice or an excursion, as well as third-year students who finish earlier, will receive the corresponding number of points according to the rules. 4) The student must meet all the requirements for completing the subject (see above) and obtain the necessary points for preparation and submitted protocols. 75-100% of points: Credit is awarded. 50-75% of points: The student will write a credit test (the test must be written for more than 70% of the points to be awarded credit, the credit test can only be repeated once). < 50% of points: The student will not be awarded credit, he must re-enroll in the course next year. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Chemistry for Education (2019) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |