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Lecturer(s)
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Sedlářová Michaela, doc. RNDr. Ph.D.
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Course content
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Lectures: The role of microorganisms in nature. Microbiology as a science: scope of the research field, its historical overview and personalities. The microbial cell structure: focused on bacteria. Nutrition and metabolism of bacteria (fermentation, respiration, photosynthesis). Growth and cultivation of microorganisms, influence of environmental factors, compounds with antimicrobial activity and mechanisms of their effect. Genetic information, its transfer and potential for biotechnological applications, bakteriophages, CRISPR. Proteosynthesis - its characteristics in bacteria. Microbial ecology, the importance of microorganisms in nutrient cycling, and bioremediations. Mechanisms of symbiosis and pathogenesis, the interactions of microorganisms with humans, animals and plants. Bacteriophages. Taxonomy and phylogeny of Archea and Bacteria, characteristics of important microbial groups and taxa. Species of economic importance. Practicals: routine methods used for isolation, cultivation and identification of microorganisms.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook), Observation, Demonstration, Projection (static, dynamic), Training in job and motor Skils, Laboratory Work
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Learning outcomes
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The aim is to introduce microorganisms - their biological characteristics, environmental conditions and techniques of cultivation, practical utilization, roles in ecosystems, human health, etc.
After attending the course a student should be able to: - construct a timeline of significant events in the history of microbiology and human diseases, indicate outstanding personalities and their contribution to development of microbiology - select and employ proper methods for determination of microorganisms in samples from various environments (water, air, soil, plant material, medicinal samples) - select staining methods and microscopy techniques to study cell structure - modify cultivation guidelines according to biology of microorganisms and their environmental requirements - apply principles of disinfection/sterilization during growth media preparation, inoculation and cultivation to avoid overgrowth of microorganisms in axenic cultures - explain the interactions of microorganisms with other (micro)organisms, and connection to human health - apply knowledge on bacterial genetics in plant biotechnology
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark, Oral exam, Written exam
Credit: 100% attendance in practicals, test, protocols Exam: knowledge from both lectures and practicals (combined exam - test + oral)
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Recommended literature
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Bednář et al. (1996). Mikrobiologie lékařská.
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Klaban V. (1999). Svět mikrobů. Hradec Králové.
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Kopecká, J., Rotková, G. (2017). Skripta ke cvičení z obecné mikrobiologie, cytologie a morfologie bakterií. Elportál, Brno: Masarykova univerzita.
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Němec M.,Horáková D. (1993). Základy mikrobiologie. Brno.
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Prescott, L.M., Harley, J.P., Klein, D.A. (2005). Microbiology. 6th edition. McGraw-Hill.
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Rosypal S. (1981). Obecná bakteriologie. SNP,Praha.
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Willey, JM, Sherwood, LM, Woolverton, CJ. (2009). Prescott´s Principles in Microbiology. McGraw-Hill.
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