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Lecturer(s)
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Drahoš Bohuslav, doc. RNDr. Ph.D.
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Course content
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1. History and development of the nuclear magnetic resonance spectroscopy, theoretical background of the method 2. Parameters of 1D NMR spectra chemical shift, signal splitting (coupling constant), integral intensity 3. Studied nuclei, description of FT NMR spectrometer and sample, measuring procedure 4. Methodology of NMR experiment, pulse sequences, FID, data processing 5. Spectra analysis - interpretation of 1H and 13C NMR spectra and elucidation of molecular structure (practical examples) 6. Selected 1D NMR techniques relaxation, dynamic effects, multiple resonance and decoupling, Nuclear Overhauser Effect, spectral editing spin echo, polarization transfer, inverse detection, pulsed field gradients 7. Basics of 2D NMR spectroscopy homonuclear and heteronuclear 2D experiments (COSY, TOCSY, HETCORE, HMQC, HSQC, HMBC) 8. Spectroscopy of other common nuclei 9. Solid-state NMR spectroscopy and MRI
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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The objective of the course is to help the students to understand basic principles of nuclear magnetic resonance, instrumental equipment and modern one- and multi-dimensional techniques of NMR experiments. Furthermore, some applications of NMR are illustrated. Essential practical part of this course consists of interpretation of NMR spectra and consequent determination of molecular structure of basic molecules.
Students have knowledge of basic concepts and laws of NMR spectroscopy. They describe the functions of NMR spectrometer and applications of this method in chemistry.
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Prerequisites
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Master's degree in Chemistry (or related field of study).
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Assessment methods and criteria
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Oral exam
For successful completion, the student must be able to answer the questions in the discussion.
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Recommended literature
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Breitmaier, E. (2002). Structure Elucidation by NMR in Organic Chemistry: A Practical guide. John Wiley & Sons, Chichester, England.
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Duer, M. J. (2010). Introduction to Solid-State NMR Spectroscopy.
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Friebolin, H. (2005). Basic One- and Two-Dimensional NMR Spectroscopy. Wiley VCH, Weinheim, Germany.
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Lambert, J. B., Mazzola, E. P. (2004). Nuclear Magnetic Resonance Spectroscopy. An Introduction to Principles, Applications, and Experimental Methods. Pearson Education, New Jersey, USA.
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Mitchell, T. N.; Costisella, B. NMR - From Spectra to Structures - An Experimental Approach.
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S. Braun, H. O. Kalinowski, S Berger. (1998). 150 and more basic NMR experiments: a practical course. Wiley VCH, Weinheim, Germany.
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