Course: Separation Methods 1

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Course title Separation Methods 1
Course code ACH/SEM1
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 1
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Lemr Karel, prof. RNDr. Ph.D.
  • Barták Petr, prof. RNDr. Ph.D.
Course content
Division of separation methods . Separation according to the size of particles on the basis of various migration rate in a force field (sedimentation, centrifugation, ultracentrifugation, cyclones, impactors , foam flotation, thermodiffusion, electrostatic and magnetic separations, methods of flow analysis-FFF), permeation methods, ( sieving, filtration, membrane methods, ultrafiltration, dialysis, electrodialysis, reverse osmosis ). Physical-chemical separations ( chilling, crystallization, zonic crystallization, salt displacement and precipitation, inclusive compounds, sublimation, lyophilization, distillation and rectification-, azeotropic dislillation,co-distillation, vacuum distillation, molecular distillation). Selective dissolution, (L-S extraction). Liquid (L-S ) extraction ( deviations from distributive principle, extraction system of simple molecules, pseudomolecular substance, chelate complexes and ion ). Gas Chromatography: theory -Van Deemeterś equation and characteristic of individual terms , equations for capillary and packing columns, differentiation and its influencing, column selection. Sample injection system for packing and capillary columns, TCD, FID, AFID, (TID,NPD), HeD(ArD), CSD, ECD,PID detectors ,electrochemical detectors, O-FID, combined techniques (GC/AED,GC/MS,GC/FTIR). Quality in GC-retention data, retention indexes and their connections with a structure, selective detection, derivatization, reaction gas chromatography, ( abstraction techniques, hydrogenation, dehydrogenation, carbon skeleton chromatography , pyrolysis, identification reaction behind a column). Quantification of results in column methods.

Learning activities and teaching methods
Lecture
  • Attendace - 26 hours per semester
  • Preparation for the Course Credit - 4 hours per semester
Learning outcomes
The aim is to get an overview of classical separation methods and gas chromatography.
Define main concepts, ideas and methods, outline analytical procedures and strategies in the field of separation methods.
Prerequisites
The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.

Assessment methods and criteria
Oral exam, Written exam

discussion on selected theme and colloquium
Recommended literature
  • C.F. Poole. Gas Chromatography. Elsevier, Amsterdam 2012.
  • D. Harvey, The Mc Graw Hill 2000. Modern Analytical Chemistry.
  • D.A. Skoog a kol. Analytická chemie. VŠCHT, Praha 2019..
  • Ed. R.L. Grob, E.F. Barry. Modern Practice of Gas Chromatography, Wiley-Interssience , 2004.
  • J.Churáček a kol. Analytická separace látek, SNTL Praha, 1990..
  • Poole C. F., Poole S. K. Chromatography today, Elsevier, Amsterdam 1991.
  • R.L. Grob, E.F. Barry. Modern Practice of Gas Chromatography, John Wiley & Sons, Inc., Hoboken 2004..
  • Štulík K. a kol. Analytické separační metody, Karolinum UK Praha 2004.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Nanomaterial Chemistry (2021) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Nanomaterial Chemistry (2021) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Inorganic and Bioinorganic Chemistry - specialization in Inorganic Materials (2021) Category: Chemistry courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Analytical Chemistry (2021) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Winter