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Lecturer(s)
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Havlíček Vladimír, prof. Ing. Dr.
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Course content
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Contents: 1. Introduction: Mass spectrometry in the analysis of organic molecules 2. Desorption techniques for the analysis of small polar molecules and large biomolecules 3. New modern high-resolving power mass analyzers: orbitrap and cyclotron 4. Desorption electrospray and jet dissociation for the analysis of tissues and whole blood 5. Biomarker discovery: Surface Enhanced Laser Desorption Ionization 6. Metabolomics 7. Surface Plasmon Resonance: interactions studies 8. Mass Microscopy and mass imaging: direct approach to biomarkers 9. Diagnostics of selected disorders
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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The aim is to become familiar with methods of analysis of Biomarkers by Mass Spectrometric Methods
Compare advantages and disadvantages of particular methods of biomarkers analysis using MS techniques, choose the suitable method for a given purpose, interpret results
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Oral exam, Written exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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(2007). International Journal of Mass Spectrometry, Special Issue on Imaging Mass Spectrometry, Vol. 260.
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Holčapek, M. (editor). (2001). Spojení vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie. Spektroskopická společnost Jana Marca Marci a Univerzita Pardubice, Pardubice.
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Jegorov, A., Hajduch, M., Sulc, M., Havlicek, V. (2006). Nonribosomal cyclic peptides: specific markers of fungal infections. J. Mass Spectrom., 41: 563.
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Kinter, M.,Sherman N. E. (2000). Protein Sequencing and identification using tandem mass spectrometry. John Wiley and sons, New York.
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Skládal, P., Macholán, L. (1997). Biosensory - Současný stav a perspektivy. Chem. Listy 91; 105-113.
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Snyder, A. P. (2000). Interpreting protein mass spectra. American Chemical Society and Oxford University Press, Washington D.C.
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