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Lecturer(s)
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Lemr Karel, prof. RNDr. Ph.D.
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Course content
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Development of theoretical knowledge describing behavior of compounds during their mass spectrometric analysis. New approaches to ionization of analytes (DESI, DART...), advances in separation of ions (orbitrap, combination of mass analyzer with ion mobility separation...). Use of mass spectrometry in different application fields, e.g. drug development, metabolomics, biomarkers, clinical application, food analysis, forensic and security application.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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The aim is to provide deeper insight into principles and applications of mass spectrometry, information concerning new knowledge and to develop student´s abilities to solve research tasks and routine applications of mass spectrometry. Attention is focused on theory describing behavior of analytes in the course of mass spectrometric analysis (ionization of analytes, separation of ions, their fragmentation), on instruments (ion sources, mass analyzers, ion detection, vacuum systems) and data interpretation. Student acquaints with new procedures and techniques in mass spectrometric analysis (e.g. ambient ionization, mass spectrometry imaging, development of mass analyzers, ion mobility-mass spectrometry, miniaturization of mass spectrometers), with application of mass spectrometry in various fields, e.g. drug development, metabolomics, biomarkers, clinical applications, food analysis, forensic and security applications, analysis of art objects
To explain actual trends in mass spectrometry, on the basis of deeper knowledge of basic principles to choose a suitable procedure for a mass spectrometric analysis and to interpret obtained data.
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Oral exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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Technická a aplikační sdělení předních výrobců hmotnostních spektrometrů.
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Barker J. (1999). Mass spectrometry. John Wiley and sons, Chichester.
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Cole R. B. (editor). (1997). Electrospray ionization mass spectrometry. Wiley Chichester.
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de Hoffmann E., Stroobant, V. (2002). Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester.
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Holčapek, M. (editor). (2001). Spojení vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie. Spektroskopická společnost Jana Marca Marci a Univerzita Pardubice, Pardubice.
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Niessen, W. M. A. (1999). Liquid Chromatography-Mass Spectrometry. Marcel Dekker, New York.
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Původní vědecká sdělení věnovaná novým poznatkům v teorii, novým trendům v instrumentaci a zajímavým aplikacím. Analytical chemistry, Journal of the American society for mass spectrometry, Journal of mass spectrometry, Mass spectrometry review...
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Ubik K. (2000). Fyzikálně-chemické metody, část 2, Hmotnostní spektrometrie. Ústav organické chemie a biochemie AV ČR, Praha.
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Watson J. T. (1997). Introduction to Mass spectrometry. Lippincott-Raven Publishers, Philadelphia.
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