Course: Microtechniques in Analytical Chemistry

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Course title Microtechniques in Analytical Chemistry
Course code ACH/MTAC
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 2
Language of instruction Czech, English
Status of course Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Petr Jan, doc. RNDr. Ph.D.
Course content
1. Microscopy, fluorescence microscopy (chemical reactions, immunochemical staining, reconstruction), histochemistry 2. Flow cytometry, laser microdissection 3. Atomic force microscopy, scanning probe microscopy, Raman spectrometry, mechanical nanoscale operations 4. Sensors, microsensors, nanopores, stochastic sensing 5. Micrototal analytical systems (microTAS), lab-on-a-chip, microfluidics, physics of microsystems 6. Microfluidic devices - materials and technologies 7. Microfluidic devices - hydrodynamic and electrokinetic flow, injection, separation 8. Microfluidic devices - detection, mass spectrometry, regulation 9. Applications of microfludic devices - droplet chemistry, PCR, sequenators, proteomics 10. Applications of microfludic devices - diagnostics, immunochemistry 11. Low cost chips (paper technology), electrowetting on dielectrics, digital microfluidics 12. Nanoparticles and microparticles in analytical chemistry

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
  • Attendace - 26 hours per semester
  • Preparation for the Exam - 34 hours per semester
Learning outcomes
The aim of this course is to introduce to students modern microscale analytical techniques and miniaturized analytical techniques.
to describe basic principles of miniaturized techniques in analytical chemistry and their application possibilities
Prerequisites
The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.

Assessment methods and criteria
Oral exam, Written exam

The examination is done by a test (minimally 60 % points should be correct) and by an oral colloquium on two optional themes.
Recommended literature
  • C. H. Li. (2005). Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery. CRC Press.
  • E. M. Goldys. (2009). Fluorescence Applications in Biotechnology and Life Sciences. Wiley-Blackwell.
  • J. P. Landers. (2007). Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques. CRC Press.
  • P. Tabeling. (2006). Introduction to Microfluidics. Oxford University Press.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biotechnology and Genetic Engineering (2019) Category: Chemistry courses - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Bioorganic Chemistry and Chemical Biology (2020) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Analytical Chemistry (2021) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Physical Chemistry (2021) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer